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Detection of CD1d and MHC class II expression on the adipocytes by immunofluorescence. (Top) A section of AT was stained with anti-CD1d antibody followed by AF488-conjugated goat anti-mouse IgG. (Bottom) A section of AT was stained with anti-MHC class II antibody followed by AF647-conjugated goat anti-mouse IgG. Images show localization of CD1d or MHC class II on adipocytes, alone or together with DAPI. Results are representative of 8 independent experiments.
