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Relative transcript analysis of galE (A) and galK (B) genes affected by the presence of dCas9-crRNA complexes. galE and galK transcripts were analyzed for monitoring of transcriptional initiation and elongation, respectively. The relative abundance of galE and galK transcripts was obtained by calculating the normalized transcript data of galE and galK genes in the presence of L-arabinose (dcas9 induced) divided by those in the absence of L-arabinose (dcas9 uninduced).
