TABLE 2.
Primers used in this study.
| Name | Sequence (5′→3′) | Description |
| Cas-AraC-F1 | GCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTTTGG | Construction of cas9-KmR cassette |
| ATGGAGTGAAACGATGGATAAGAAATACTCAATAGGCT | ||
| KmR-AraD-R4 | GTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGT | Construction of cas9-KmR cassette |
| GGCGTGGCGCGAGTGTAGGCTGGAGCTGCTTCGAAGTT | ||
| Cas-KmR-OR2 | GACGGATCCCCGGAATTCAGTCACCTCCTAGCTGACTCAAA | Construction of cas9-KmR cassette |
| Cas-KmR-OF3 | GCTAGGAGGTGACTGAATTCCGGGGATCCGTCGACCTGCAG | Construction of cas9-KmR cassette |
| Ara_F1 | AGCAGCTCCGAATAGCGCCCTTCCCCTTGC | Confirmation of ΔaraBAD |
| AraD_500dn | CCAGCCAGAAGGAGACTTCTGTCCCTTG | Confirmation of ΔaraBAD |
| Sm_ATG_R | GATACTGGGCCGGCAGGCGCTCCATTGCCC | Construction of crRNA plasmids |
| Sm_TAA_F | GCAATGGAGCGCCTGCCGGCCCAGTATCAG | Construction of crRNA plasmids |
| pTarget_seq | AACGCCTGGTATCTTTATAGTCCTGTCG | Sequencing of crRNA plasmids |
| Cas-galK-WF | AGGCTGTAACTGCGGGATCAGTTTTAGAGCTAGAAATAGCAAG | Construction of pHK459 plasmid |
| Cas-galK-WR | TGATCCCGCAGTTACAGCCTACTAGTATTATACCTAGGACTG | Construction of pHK459 plasmid |
| galT_Target_F | CGAACAGAAATCACCAATGCGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ003 plasmid |
| galT_Target_R | GCATTGGTGATTTCTGTTCGACTAGTATTATACCTAGGACTG | Construction of pBJ003 plasmid |
| galE Target_F | TACGTTGAAAGCTTCCCGACGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ004 plasmid |
| galE_Target_R | GTCGGGAAGCTTTCAACGTAACTAGTATTATACCTAGGACTG | Construction of pBJ004 plasmid |
| Pgal Target_F | TTCGCATCTTTGTTATGCTAGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ005 plasmid |
| Pgal Target_R | TAGCATAACAAAGATGCGAAACTAGTATTATACCTAGGACTG | Construction of pBJ005 plasmid |
| galK_CAG_F | GTAACTGCGGGATCATGGATGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ021 plasmid |
| galK_CAG_R | ATCCATGATCCCGCAGTTACACTAGTATTATACCTAGGACTG | Construction of pBJ021 plasmid |
| galK_GCG_F | CCAGTTTGTAGGCTGTAACTGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ022 plasmid |
| galK_GCG_R | AGTTACAGCCTACAAACTGGACTAGTATTATACCTAGGACTG | Construction of pBJ022 plasmid |
| galK_ATG_F | TAGGCTGTAACTGCGGGATCGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ023 plasmid |
| galK_ATG_R | GATCCCGCAGTTACAGCCTAACTAGTATTATACCTAGGACTG | Construction of pBJ023 plasmid |
| galK_GGA_F | GGCTGTAACTGCGGGATCATGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ024 plasmid |
| galK_GGA_R | ATGATCCCGCAGTTACAGCCACTAGTATTATACCTAGGACTG | Construction of pBJ024 plasmid |
| galK_AGC_F | TAACTGCGGGATCATGGATCGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ025 plasmid |
| galK_AGC_R | GATCCATGATCCCGCAGTTAACTAGTATTATACCTAGGACTG | Construction of pBJ025 plasmid |
| galK_TGT_F | CAGAAAACCAGTTTGTAGGCGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ026 plasmid |
| galK_TGT_R | GCCTACAAACTGGTTTTCTGACTAGTATTATACCTAGGACTG | Construction of pBJ026 plasmid |
| Pgal_AAG_F | GAAATAACCATAGCATAACAGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ027 plasmid |
| Pgal_AAG_R | TGTTATGCTATGGTTATTTCACTAGTATTATACCTAGGACTG | Construction of pBJ027 plasmid |
| Pgal_GCG_F | AACCATAGCATAACAAAGATGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ028 plasmid |
| Pgal_GCG_R | ATCTTTGTTATGCTATGGTTACTAGTATTATACCTAGGACTG | Construction of pBJ028 plasmid |
| Pgal_ATG_F | TTTCGCATCTTTGTTATGCTGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ029 plasmid |
| Pgal_ATG_R | AGCATAACAAAGATGCGAAAACTAGTATTATACCTAGGACTG | Construction of pBJ029 plasmid |
| Pgal_AGA_F | AAATAACCATAGCATAACAAGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ030 plasmid |
| Pgal_AGA_R | TTGTTATGCTATGGTTATTTACTAGTATTATACCTAGGACTG | Construction of pBJ030 plasmid |
| Pgal_TGC_F | TAACCATAGCATAACAAAGAGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ031 plasmid |
| Pgal_TGC_R | TCTTTGTTATGCTATGGTTAACTAGTATTATACCTAGGACTG | Construction of pBJ031 plasmid |
| Pgal_GGT_F | TCGCATCTTTGTTATGCTATGTTTTAGAGCTAGAAATAGCAAG | Construction of pBJ032 plasmid |
| Pgal_GGT_R | ATAGCATAACAAAGATGCGAACTAGTATTATACCTAGGACTG | Construction of pBJ032 plasmid |
| galE-RTL | CCACCGTTTATGGCGATCAG | RT-qPCR for transcripts of galE gene |
| galE-RTR | GTTCCACCATCAGCTTGCTT | RT-qPCR for transcripts of galE gene |
| galK-RTL | GCCTCTATGCGCGATGATTT | RT-qPCR for transcripts of galK gene |
| galK-RTR | CACCTTTGTCGCCAATCACA | RT-qPCR for transcripts of galK gene |
| 16S-RTL | CAGCAGCCGCGGTAATAC | RT-qPCR for 16S rRNA |
| 16S-RTR | ACCAGGGTATCTAATCCTGT | RT-qPCR for 16S rRNA |