FIGURE 7.

The hydrolysis activity of B. subtilis Rel is not activated by branched-chain amino acids binding to the RRM domain. (A) Hydrolase activity of B. subtilis Rel is not stimulated by leucine. The experiments were performed in HEPES:Polymix buffer, pH 7.5 at 37°C in the presence of 5 mM Mg²⁺. Error bars represent standard deviations of the turnover estimates by linear regression using four data points. (B) Sequence alignment of the B. subtilis N685 region of representative long RSHs. (C,D) Wild-type B. subtilis 168 and isogenic chromosomal relN685A mutant (VHB455) were grown on solid (C) and liquid (D) LB media at 37°C. The plate was scored after 12 h at 37°C. The growth rates (μ²) were calculated from three independent biological replicates and the error bars (shown as shadows) represent standard deviations. Since the liquid media growth experiments were performed in plates format in Bioscreen C growth curve analysis system the OD₆₀₀ is presented in arbitrary units.