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. 2019 Nov 6;25(21-22):1493–1503. doi: 10.1089/ten.tea.2018.0371

FIG. 1.

FIG. 1.

Characterization of human AFSC. (A) Fibroblast-like cell morphology of AFSC at 48 h after seeding. Scale bar: 100 μm. (B) Cell growth curve of AFSC at P13. (C) Phenotypic characterization of AFSC by flow cytometric analysis. Representative histograms showing positive expression of SSEA4, Oct4, CD90, CD44, CD73, CD105 at 13th passage. The respective isotype control is shown as a green line, except for Oct4. (D) Multidifferentiation capability of AFSC showing adipogenic and osteogenic differentiation, revealed by formation of lipid droplets (arrowheads) and Alizarin Red staining, respectively. Scale bar: 100 μm. AFSC, amniotic fluid stem cell. Color images are available online.