Figure EV4. Overexpression of HuR alone failed to prevent the anti‐inflammatory response caused by the drug‐resistant parasite Ld ^R .

• A, B
  Internalization of Ld (Ld ^R and Ld ^S) in RAW 264.7 cells. Cells were pre‐transfected with HA‐HuR expression plasmid and infected with CFSE labelled Ld ^R parasite (with 1:10 host:parasite ratio) for 24 h, and parasite internalization was imaged using confocal microscope (A). HA‐HuR‐transfected cells were stained for HA (red), and parasites were labelled with CFSE dye (green) prior to infection. DAPI was used to label nuclei. Number of infected cells was counted from control and HA‐HuR‐expressing cells (B). Values are mean ± s.e.m., n = 6.
• C
  Expression of cytokines in cells infected with Ld ^R strain of Ld. Cytokine mRNA levels like TNF‐α and IL‐1β were quantified by real‐time PCR from pCIneo or HA‐HuR‐transfected RAW 264.7 cells after 24‐h infection with Ld ^R parasites mean ± s.e.m., n = 3.

Data information: For statistical analysis, all experiments are done three times and the P‐values were calculated using Student's t‐test. Exact P‐values against each experimental set are presented in the Appendix Table S2.