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. 2019 Autumn;18(4):1659–1675. doi: 10.22037/ijpr.2019.1100872

Figure 2.

Figure 2

(A) The pGE-GFP plasmid containing GFP gene and cis-acting flanking structures such as T7 promoter, the 5 and 3un-translated regions (UTRs) adjacent the ORF. At the beginning and the end of the sequence containing the GFP gene, two restriction enzymes Hindlll and Gsul have been placed. (B) The GFP mRNA transcript with a length of about 1.6 kb shown on an agarose gel 1.1%. Marker Column: RNA marker with a length of 0.5-9 kb