Figure 9.

Flow cytometry analysis of moDCs viability, before and after transfection with NPs. The dot plots show Near-IR fluorescence on the x-axis and count cells on the y-axis. Gates were drawn based on un-staining Mo-DCs (row cells). Percentages of dead cells (left corner) and viable cells (right corner) are indicated. (A) The percent viability of iDC calls before transfection with PLGA/PEI nanoparticles. (B-D) The percent viability of moDC calls after transfection with PLGA/PEI nanoparticles at 12, 24 and 48 h after exposure respectively. (E) Comparison of the mean viability of moDCs cells before and after exposure to nanoparticles (at 12, 24, 48 h, respectively). Measurements were repeated in triplicate for each time and the standard errors are shown