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. 2020 Feb 28;11:143. doi: 10.3389/fphar.2020.00143

Figure 2.

Figure 2

Decreased membrane CaM in HNSCC T cells. (A) Representative confocal images of HD and HNSCC T cells stained for CD8 (green; no permeabilization) and CaM (magenta, after permeabilization). Scale bars indicate 10 μm. Representative individual cells highlighted (box) in the merged images are magnified in the extreme right panels. Co-localization is indicated by white color. (B, C) Fluorescence intensities of CaM in the membrane (B) and cytoplasm (C) of HD and HNSCC T cells were measured as described in Materials and Methods. Data represented by a box and whisker plot. The horizontal line represents median value of CaM fluorescence intensities for each group, while the first and third quartiles are represented in the top and bottom boxes respectively. (D) Ratio of CaM fluorescence at the cell membrane and cytoplasm of HD and HNSCC T cells. Bars represent mean ± SEM (E) Representative confocal images of HD and HNSCC T cells stained for KCa3.1 (green). Scale bars indicate 10 μm. (F) Fluorescence intensities of KCa3.1 in the membranes of HD and HNSCC T cells. Bars represent mean ± SEM of 117 cells from 3 HD and 61 cells from 3 HNSCC patients. Shown in (B–D) are values for 96 cells from 3 HD and 110 cells from 3 HNSCC patients. Data in (B–D, F) were analyzed by Mann Whitney rank sum test.