Figure 2.

Evolution of Fibroblast States across Phases of Repair

(A) t-SNE plot of combined stromal clusters (fibroblasts I, II, and III, myofibroblasts) and epicardium, further sub-clustered by 20 PC, resolution 0.5, colored by cluster separation.

(B) Bar plot representation of the relative frequency of different fibroblast sub-clusters (percentage over total cells) at different time points across repair.

(C) Dot-plot representation of top two signature genes per sub-cluster.

(D) SPRING visualization of fibroblast sub-clusters; 1,200 cells are shown per time point, colored by Seurat-defined clusters.

(E) PANTHER GO-slim biological process overrepresentation analysis of fibroblasts sub-cluster marker genes, showing representative GO terms per each sub-cluster, excluding DCs, dendritic-like cells, and IFNr (interferon-responsive cells). Bonferroni corrected p values.

See also Figures S2–S4 and Tables S3 and S4.