Figure 3.

Endocardial-Derived Fibroblast (EndD) Validation in Homeostatic Ventricles

(A) Confocal imaging of the EndD marker DKK3 (in red) on cardiac sections from adult Wt1^Cre; ZsGreen^f/+ mice. ZsGreen labels epicardial derived cells.

(B) Quantification of the percentage of DKK3⁺ cells co-labelled with the reporter ZsGreen. Data are represented as mean ± SEM. N = 3 biological replicates, 7 technical replicates.

(C) Confocal imaging of DKK3 (in red) on cardiac sections from adult Tek^Cre; ZsGreen^f/+ mice. ZsGreen labels endocardial-derived cells.

(D) Quantification of the percentage of DKK3⁺ cells co-labelled with the reporter ZsGreen. Data are represented as mean ± SEM. N = 3 biological replicates, 7 technical replicates.(E) Enrichr analysis of EndD marker genes identified top related cell (mouse gene atlas) and tissue (ARCHS4 tissues) types.

See also Figure S4 and Tables S3 and S4.