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. 2020 Feb 28;7:25. doi: 10.3389/fmolb.2020.00025

FIGURE 1.

FIGURE 1

LasR DNA-binding capacity requires 3OC12-HSL during protein expression. E. coli SHA011 LasR producing strain was grown in the presence or absence of 20 μM 3OC12-HSL. As a control, E. coli SHA010, LasR non-producing strain was grown in same conditions. Protein extracts were tested for their capacity to bind to the lasI* probe by EMSA assays in the presence of 10 μM 3OC12-HSL. As shown, the presence of 3OC12-HSL in the culture is essential for purifying an active form of LasR (able to bind to the lasI* probe) from the E. coli SHA011 LasR producing strain. A non-specific retarded band was also observed in extracts obtained either from the control strain that does not produce LasR (SHA010) or from the SHA011 LasR-producing strain.