FIGURE 3.

Partial inhibition of LasR binding activity by naringenin. The EMSA assays were carried out using a purified active protein obtained from SHA011 strain grown in the presence of 3OC12-HSL. The capacity of naringenin to displace 3OC12-HSL from LasR was analyzed by adding increasing concentrations of the flavonoid (10, 50, 500 μM) to the binding reaction. The figure shows that neither 3OC12-HSL (10 μM) nor naringenin (10 μM) addition to the binding buffer alters the LasR-DNA binding complex. However, increased concentrations of naringenin (50 and 500 μM) in the binding buffer were able to partially inhibit the LasR-DNA interaction, even in the presence of 3OC12-HSL (10 μM), evidencing that the flavonoid may partially displace the 3OC12-HSL from LasR.