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. 2020 Feb 28;7:25. doi: 10.3389/fmolb.2020.00025

TABLE 2.

Primers used in this study.

Primer Sequence Utilization
rplU.fw 5′-CGCAGTGATTGTTACCGGTG-3′ Real time PCR for rplU and for checking DNA in RNA samples
rplU.rv 5′-AGGCCTGAATGCCGGTGATC-3′
lasA.fw 5′-ATGGACCAGATCCAGGTGAG-3′ Real time PCR for lasA
lasA.rv 5′-CGTTGTCGTAGTTGCTGGTG-3′
lasB.fw 5′-ATCGGCAAGTACACCTACGG-3′ Real time PCR for lasB
lasB.rv 5′-ACCAGTCCCGGTACAGTTTG-3′
lasI.fw 5′-TGCTCTGATCTTTTCGGA-3′ Amplication and labeling of lasI*
lasI.rv 5′-CGATCATCTTCACTTCCT-3′ probe used in EMSA
lasR-pGEX.fw 5′-CCGGATCCATGGCCTTGGTTGACGGTTT-3′ LasR amplification for cloning in pGEX6p expression vector
lasR-pGEX.rv 5′-CCGAATTCTCAGAGAGTAATAAGACCCA-3′