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. 2020 Feb 28;8:138. doi: 10.3389/fchem.2020.00138

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Copyright © 2020 Rebello, Nicolardi, Lageveen-Kammeijer, Nouta, Gardner, Mesker, Tollenaar, Spencer, Wuhrer and Falck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of core fucosylated glycans and antennary fucosylated glycans by exoglycosidase and MALDI-FT-ICR-MS. The TPNG profile without core fucosidase treatment was compared to the profile after treatment, within (A) the m/z range of 1,200–3,200 and (B) the m/z range of 3,120–4,200. Core fucosylated glycans [red m/z values] are converted to their corresponding afucosylated glycans [purple m/z values], upon core fucosidase treatment. Only the antennary fucosylated glycans [blue m/z values] and the antennary fucose isomers of the mixed fucose isomeric (core or antennary fucosylation) glycans [green m/z values] remain after core fucosidase treatment. All m/z values of annotated glycans belong to [M + Na]⁺ ions. The description of the glycan cartoons are shown in Figure 1.