Figure 5.

CE-ESI-MS/MS spectra of sialic acid derivatized and GiRP labeled TPNG for the confirmation of antennary, mixed isomeric (core or antennary fucosylation) and core fucosylated glycans. (A) The [M+H]²⁺ ion of H4N4F1 (m/z 879.838) is fragmented for fucosidase untreated TPNG. Its fragment ions are indicative of core fucosylation. (B) The [M+2H]³⁺ ion of H6N5F1E2 (m/z 975.709) [second isotope] is fragmented for fucosidase untreated TPNG. Its fragment ions are indicative of both core fucosylated and antennary fucosylated isomers. (C) The ion [M+2H]³⁺ of H6N5F1E2 (m/z 975.709) [second isotope] is again fragmented, but for core fucosidase treated TPNG. Its fragmentation pattern is indicative of only antennary fucosylation. The descriptions of the glycan cartoons are shown in Figure 1. GiRP represents Girard's reagent P label; * represents an oxonium ion.