Table 2.

Comparison of the homology of myostatin (MSTN) protein sequences in Nile tilapia with other species.

Protein	Nile tilapia 1	Nile tilapia 2	Human	Mouse	Cow	Salmon 1a	Salmon 1b	Salmon 2a	Medaka	Zebrafish 1(b)	Zebrafish 2(a)
Nile tilapia 1		69	66	65	63	83	82	66	86	80	64
Nile tilapia 2	82		60	63	63	67	66	76	68	71	67
Human	80	74		96	94	66	66	59	64	68	64
Mouse	79	76	98		93	65	65	59	63	67	63
Cow	78	76	96	95		64	64	58	62	66	63
Salmon 1a	90	77	78	78	77		93	66	83	86	65
Salmon 1b	91	76	79	79	77	95		68	80	85	66
Salmon 2a	80	86	74	73	73	77	79		64	66	64
Medaka	92	79	77	76	75	88	88	77		80	64
Zebrafish 1(b)	90	82	82	81	76	90	91	78	87		70
Zebrafish 2(a)	78	80	77	77	80	78	78	80	77	82

Table comparing of human, mice, cattle, salmon, Medaka, and zebrafish. Percentage of identity (in bold) and of similarity are shown of protein sequences of Nile tilapia (Oreochromis niloticus) myostatin1 (accession number XP_003458880); Nile tilapia myostatin2 (MN708486); human (Homo sapiens) growth/differentiation factor 8 preproprotein (NP_005250); mouse (Mus musculus) growth/differentiation factor 8 preproprotein (NP_034964); cattle (Bos taurus) growth/differentiation factor 8 precursor (NP_001001525); salmon (Salmo salar) myostatin 1a (ABN72586); salmon myostatin 1b precursor (NP_001117106); salmon myostatin 2a (ABN72587); medaka (Oryzias latipes) growth/differentiation factor 8 precursor (NP_001188428); zebrafish (Danio rerio) myostatin a precursor (NP_001004122); and zebrafish growth/differentiation factor 8 preproprotein (NP_571094). Salmon myostatin 2a is a pseudogene, hence it is not translated into a protein. Regions of local similarity between the protein sequences above were identified using BLAST.