TABLE 1.

Male baseline faecal glucocorticoid metabolite concentrations, as well as the mean absolute deviation from baseline, for all three enzyme immunoassays employed during the study.

Male (EIA)	Baseline fGCM (fGCM concentration)	Physiological stressor ACTH injection		Biological stressor Injury and separation
		Peak fGCM concentration (hours post-injection)	Peak response (%)	Peak fGCM concentration (hours post-injection)	Peak response (%)	Period of elevated fGCM concentrations (hours post-injection)
11,17 DOA	0.95	1.15 (23)	21.33	3.51 (96)	369.47	71.5–103
3a,11oxo-CM	1.16	1.98 (23)	71.09	3.46 (96)	198.28	71.5–191.5
cortisol	0.08	0.18 (23)	129.17	0.14 (103)	70.89	N/A

Note: The peak faecal glucocorticoid metabolite concentration, as well as the peak response (%) from the calculated baseline level, for the described physiological stressor, are shown. For the biological stressor, the peak faecal glucocorticoid metabolite concentration and time until the peak sample (post-injection) is shown. Additionally, the peak response, in per cent increase from baseline faecal glucocorticoid metabolite value, as well as the period of elevated faecal glucocorticoid metabolite levels during the biological stressor period, is indicated for each enzyme immunoassay. All faecal glucocorticoid metabolite values are in µg/g dry weight.

11,17 DOA, 11-oxoetiocholanolone I; 3a,11oxo-CM, 11-oxoetiocholanolone II.

ACTH, adrenocorticotropic hormone; fCGM, faecal glucocorticoid metabolite; EIA, enzyme immunoassay.