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. 2020 Mar 6;22:42. doi: 10.1186/s13075-020-2130-5

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — TGFβ regulates the expression of TGFβ-activating integrins in SSc monocytes. CD14+ monocytes were isolated using positive selection from the PBMC fraction of fresh blood and stimulated for 0, 1, 2, 4, 6, and 16 h with 5 ng/ml rhTGFβ1 or the TGFβ receptor inhibitor SB-505124 at a dose of 5 μM or vehicle control (DMSO) while seeded on plastic. At indicated time points, RNA was isolated and gene expression was measured