Table 1.
Gene ID | Wild-type and mutant sequences of the i-motif, G4 and hairpina | Used for |
---|---|---|
BmPOUM2-WT | TTGTTGCCCCGCCCCTCGGCCCCCTCGCGCCCCGCACTGG | BmPOUM2-WT in Fig. 1a; ssDNA, i-motif and cold probes in Fig. 2a; i-motif in Fig. 2c, Fig. 2e, Fig. 2g and Fig. 3A(a,b) and Fig. 3C; ssDNA in Fig. 2e. |
BmPOUM2-Mut | TTGTTGTACTGCTACTCGGATCACTCGAGCATCGTACTGG | Mutant probes in Figs. 1b, 2a |
BGIBMGA003213-WT | CCCCGCGCCCCCCGCGTCCCCCGCACCCCC | BGIBMGA003213-WT in Fig. 1c; ssDNA, i-motif and cold probes in Fig. 2b; i-motif in Figs. 2d,f, 3B(a,b), D; ssDNA in Fig. 2f. |
BGIBMGA003213-Mut | CATTGCGATATCAGCGTTATCAGTATCAAT | Mutant probes in Figs. 1d, 2b |
BmPOUM2-G4 | CCAGTGCGGGGCGCGAGGGGGCCGAGGGGCGGGGCAACAA | BmPOUM2 G4 in Fig. 2e |
BGIBMGA003213-G4 | GGGGGTGCGGGGGACGCGGGGGGCGCGGGG | 3213 G4 in Fig. 2f |
Hairpin | CAGTGGCGGCCATCCGTTTAATATTACCGGATGGCCGCGCGAT | Hairpin in Fig. 2e, f |
Hairpin was synthesized based on the tomato golden mosaic virus sequence. The i-motif, G4 and mutant sequences were synthesized based on the sequences of BmPOUM2 and BGIBMGA003213 genes
aThe underlined based are mutated