Table 3.
Description of the most used software for bone marrow histomorphometry.
| Software | Species, sample | Automatic or manual | Other methods | Reported parameters | Proposed parameters | Other measures | Stains (embedding) | References |
|---|---|---|---|---|---|---|---|---|
| OsteoMeasure | Monkey, proximal femur | Manual | – | – | Ad.Ar/T.Ar | – | – | (32) |
| OsteoMeasure | Human, biopsy | Manual | Blinded count | N.Ad, % Ad.V /TV (AV/TV); total Ad.Pm | ΣAd.Ar/T.Ar; N.Ad/T.Ar; total Ad.Pm |
– | Goldner, 20x | (33) |
| OsteoMeasure | Mouse, femur | Manual | – | AV/TV; Ad.Pm; N.Ad/T.Ar. | ΣAd.Ar/T.Ar; Ad.Pm; N.Ad/T.Ar |
Bone standard histomorphometry | Goldner's Trichrome/Von Kossa | (34) |
| OsteoMeasure | Mouse, distal femur | Manual | – | N.Ad/T.Ar | N.Ad/T.Ar | Bone standard histomorphometry | – | (35) |
| OsteoMeasure | Proximal tibia metaphysis | Manual | – | – | N.Ad/T.Ar | Bone standard histomorphometry | unstained (paraffin) | (36) |
| OsteoMeasure | Mouse, femur | Semi-automatic | – | Marrow fat content % (AV/TV) | ΣAd.Ar/T.Ar | Bone standard histomorphometry | H&E | (37) |
| OsteoMeasure | Mouse, tibia | manual | – | N.Ad/T.Ar; Ad.Dm |
N.Ad/T.Ar; Ad.Dm |
Bone standard histomorphometry | TRAP/toluidine blue | (38) |
| OsteoMeasure | Mouse, distal femur | Manual | – | Ad.Ar/T.Ar; N.Ad/T.Ar |
Ad.Ar/T.Ar; N.Ad/T.Ar |
Bone standard histomorphometry | TRAP/toluidine blue | (39) |
| OsteoMeasure | Mouse, distal femur metaphysis | Manual | Fat extraction and analysis | Ma. adiposity; Ad. Density | ΣAd.Ar/T.Ar; ΣAd.Ar/Ma.Ar; N.Ad/Ma.Ar |
Bone standard histomorphometry | Methyl-methacrylate | (40) |
| OsteoMeasure | Human, bone biopsy | Manual | – | Ad.Ar/Ma.Ar | Ad.Ar/Ma.Ar | Bone standard histomorphometry | unstained, (plastic) 10x | (41) |
| OsteoMeasure | Mouse, tibia | Manual | – | N.Ad/T.Ar Ad.Ar/T.Ar | N.Ad/T.Ar; Ad.Ar/T.Ar |
Bone standard histomorphometry | H&E | (42) |
| OsteoMeasure | Mouse, distal femur | Manual | – | Ad.V/TV | ΣAd.Ar/T.Ar | Bone standard histomorphometry | – | (43) |
| Image Pro | Mouse, distal femur | Manual | – | N.Ad; Ad.Ar | Ad.Ar/T.Ar; N.Ad/Ma.Ar |
– | H&E | (44) |
| Image Pro Plus | Mouse, tibia | Manual | – | – | ΣAd.Ar/T.Ar; Ad.Ar/Ma.Ar; N.Ad/Ma.Ar |
– | H&E | (45) |
| Image Pro Plus | Mouse, tibia | Manual | – | Ad.Ar | Ad.Ar/Ma.Ar | Bone standard histomorphometry | H&E | (46) |
| Image Pro Plus v6 | Rat, femur | Manual | Manual count | N.Ad; Ad.Ar; Ad.Dm, Ad. density |
ΣAd.Ar/T.Ar; ΣAd.Ar/Ma.Ar; N.Ad/Ma.Ar; Ad.Dm |
Bone standard histomorphometry | H&E | (47) |
| Image Pro Plus v6 | Rat, femur | Manual | – | Ad.Dm, N.Ad/Ma.Ar %Ad.Ar | Ad.Dm; ΣAd.Ar/T.Ar ΣAd.Ar/Ma.Ar; N.Ad/Ma.Ar |
Bone standard histomorphometry | H&E | (48) |
| Image Pro Plus v6 | Rabbit, distal femur | Manual | – | Ad.Dm; N.Ad/Ma.Ar; Ad.Ar/Ma.Ar |
Ad.Dm; N.Ad/Ma.Ar; ΣAd.Ar/Ma.Ar |
– | H&E | (49) |
| ImageJ | Rat, proximal tibia | Manual | – | Ad. content (Ad.Ar, T.Ar) | Ad.Ar; ΣAd.Ar/T.Ar |
– | H&E | (50) |
| ImageJ | Mouse, femur or tibia | Manual | – | Ad.V/Ma.V | ΣAd.Ar/Ma.Ar | Bone standard histomorphometry | H&E (plastic or paraffin) 20x | (51) |
| ImageJ | Rat, proximal tibia | Manual | – | N.Ad/T.Ar; Ad.Ar/T.Ar |
N.Ad/T.Ar; Ad.Ar/T.Ar |
– | H&E | (52) |
| ImageJ | Mouse, distal femoral metaphysis | Automatic/ manual |
OsteoMeasure | T.Ar adiposity, N.Ad/T.Ar, adiposity (%) | ΣAd.Ar/T.Ar; ΣAd.Ar/Ma.Ar; N.Ad/T.Ar; N.Ad/Ma.Ar |
– | Von Kossa tetrachrome | (53) |
| OsteoidHisto | Human, biopsy | Semi-automatic | – | Ad.V/TV; Ad.V/Ma.V Ad.Dm N.Ad/Ma.Ar | ΣAd.Ar/T.Ar; ΣAd.Ar/Ma.Ar; Ad.Dm; N.Ad/Ma.Ar |
Bone standard histomorphometry | Goldner's Trichrome | (54) |
| OsteoidHisto | Mouse, tibia | Semi-automatic | – | Ad.V/TV Ad.V/Ma.V Ad.Dm Ad.Dm | ΣAd.Ar/T.Ar; ΣAd.Ar/Ma.Ar; Ad.Dm; N.Ad/T.Ar |
Bone standard histomorphometry | Calcein blue/TRAP | (55) |
| Bioquant Osteo | Human, biopsy | Semi-automatic | Blinded count | N.Ad, Ad. size, | N.Ad/Ma.V; ΣAd.Ar/Ma.Ar | Bone standard histomorphometry | – | (56) |
| Bioquant Osteo | Rat, tibia | Manual | – | N.Ad/TV | N.Ad/T.Ar | Bone standard histomorphometry | Goldner's trichrome | (57) |
| Bioquant Osteo II | Mouse, femur | No information | – | AV/TV | ΣAd.Ar/T.Ar | Bone standard histomorphometry | Goldner's Trichrome or TRAP | (58) |
| Leica Q-win Plus | Rabbit, vertebrae | No information | – | Ad.Dm; N.Ad/Ma.Ar | Ad.Dm; N.Ad/Ma.Ar | – | Oil-Red-O | (59) |
| MarrowQuant | Mouse, skeleton | Semi-automatic | Osmium tetroxide stain with μCT | T.Ar; Ma.Ar; N.Ad; Ad.Ar; ΣAd.Ar/Ma.Ar; Ad.V (μCT) |
T.Ar; Ma.Ar; N.Ad; Ad.Ar; ΣAd.Ar/Ma.Ar; N.Ad/Ma.Ar |
Bone Ar, hematopoietic Ar, vascular Ar | H&E (paraffin) 20x | (60, 61) |
| MarrowQuant | Mouse, tibia | Semi-automatic | – | T.Ar; Ma.Ar; Ad.Ar; ΣAd.Ar/Ma.Ar |
T.Ar; Ma.Ar; N.Ad; Ad.Ar; ΣAd.Ar/Ma.Ar; N.Ad/Ma.Ar |
hematopoietic Ar, vascular Ar | H&E (paraffin) | [Rojas-Sutterlin et al. as reviewed in (61, 62)] |
| Metamorph | Mouse, femur | Semi-automatic | – | Ad.Ar; N.Ad |
ΣAd.Ar/T.Ar; N.Ad/Ma.Ar; Ad.Dm |
– | H&E | (63) |
Ad., adipocyte; Ad.Ar, adipocyte area; Ad.Dm, adipocyte diameter; N.Ad, adipocyte number; Ar, Area; Ma., marrow; Ma.Ar, marrow area; AV, total adipocyte volume; H&E, hematoxylin and eosin; TRAP, tartrate-resistant acid phosphatase; TV, total tissue volume; Σ, summation; μCT, micro-computerized tomography.