Table 7.
Variability of in vitro murine bone marrow stromal cell adipogenic differentiation protocols.
| References | (147) | (84) | (156) | (160) | (56) (135) | (5) | |||
|---|---|---|---|---|---|---|---|---|---|
| Maintenance | |||||||||
| Medium | DMEM:F12 | α-MEM | RPMI | α-MEM | α-MEM or DMEM | 60% DMEM low Glc: 40%MCDB | |||
| Serum | 20% FBS | 10% FBS | 10% FBS | 10% FBS | 20% or 10% FBS | 2% FBS | |||
| Other | 0.1 mM NEAA | 200 μM NEAA | ITS, linoleic acid, dexa, AA, EGF, LIF, PDGFBB, bFGF |
||||||
| Adipogenic | |||||||||
| Medium | DMEM:F12 | α-MEM | DMEM | α-MEM | α-MEM or DMEM | 60% DMEM low Glc: 40%MCDB | |||
| Serum | 20% FBS | 10% FBS | 9% horse serum | 10% FBS | 20% or 10% FBS | 2% FBS | |||
| Other | 200 μM NEAA | ||||||||
| IBMX (μM) | 500 | 500 | 450 | 0.5 | 500 | 0.5 | |||
| Dexa or Hydrocortisone (μM) | 1 | 0.5 | 0.25 | 1 | 1 | 1 | |||
| Indomethacin (μM) | 100 | 60 | 50 | ||||||
| Insulin (μg/ml) | 5 | 5 | 0.01 | 10 | 10 | 10 | 5 | 5 | |
| Rosiglitazone (μM) | 1 | 1 | 1 | ||||||
| T3 (nM) | 1 | 1 | |||||||
| Differentiation time | 2 weeks | 3 weeks | 12 days | 2 weeks | 2–4 days | 3–4 days | 4 days | 48 h | 5 days |
NEAA, non-essential amino acids; dexa, dexamethasone; ITS, insulin-transferrin-selenium mix; AA, L-ascorbic acid 2-phosphate; EGF, epidermal growth factor; LIF, leukemia inhibitory factor; PDGFBB, platelet-derived growth factor BB; bFGF, basic fibroblast growth factor; FBS, fetal bovine serum.