Table 7.
Mean (SD) of terminal measurements from μCT evaluations and compression tests of the L6 VB for each group along with relevant pairwise differences, as the LS means [with SE] for significant main effects (S for p<0.05 and * indicates 0.05<p≤0.1) – Genotype (G), Age group (A), and Sex (S) – as well as significant interaction effects (GxS, GxA, and SxA). Negative value means Jimbee is higher than C57BL/6J or 49-50-weeks is higher than 16-18-weeks or Male is higher than Female. Main effects or interaction effects were not significant (NS) if p>0.1.
| FEMALE | Genotype effect1 |
Age effect2 |
Genotype Effect within Sex3 Effect within Age (Young Old)4 |
Age Effect within Sex5 Effect within Genotype (C57BL/6J Jimbee)6 |
|||||
|---|---|---|---|---|---|---|---|---|---|
| 16-18-week mice |
49-50-week mice | ||||||||
| Variable | Units | C57BL/6J (n=10) |
Jimbee (n=12) |
C57BL/6J (n=13) |
Jimbee (n=19) |
||||
| Tb.BV/TV | --- | 0.190 (0.021) | 0.202 (0.021) | 0.154 (0.020) | 0.172 (0.026) | NS | S | −0.015 [0.007], (NS) | +0.032 [0.008], (NS) |
| Conn.D | mm−3 | 151.8 (41.9) | 207.1 (35.7) | 86.2 (33.4) | 136.3 (40.7) | S | +80.0 [7.3] | −52.5 [10.1], (NS) | NS, (NS) |
| SMI | --- | 0.99 (0.17) | 0.90 (0.19) | 0.91 (0.23) | 0.95 (0.27) | NS | S | NS, (NS) | NS, (NS) |
| Tb.N | mm−1 | 4.05 (0.56) | 4.26 (0.42) | 3.03 (0.59) | 3.65 (0.95) | NS | +0.91 [0.13] | −0.46 [0.18], (NS) | NS, (NS) |
| Tb.Th | mm | 0.050 (0.002) | 0.048 (0.002) | 0.051 (0.004) | 0.049 (0.003) | +0.002 [0.001] | S | NS, (NS) | NS, (NS) |
| Tb.Sp | mm | 0.25 (0.03) | 0.24 (0.02) | 0.34 (0.06) | 0.29 (0.07) | NS | −0.06 [0.01] | +0.04 [0.01], (NS) | NS, (NS) |
| Tb.TMD | mgHA/cm3 | 975.0 (11.5) | 966.2 (9.7) | 1014.9 (19.6) | 991.1 (13.5) | S | S | +16.5 [3.8], (NS, +16.0 [3.5]) | −32.1 [3.8], (−27.7 [4.1], −15.9 [3.6]) |
| VB strength | N | 29.0 (3.7) | 30.0 (4.9) | 25.8 (3.2) | 27.5 (5.3) | NS | S | NS, (NS) | +2.8 [1.3], (NS) |
| MALE | Genotype effect |
Age effect |
Genotype Effect within Sex Effect within Age (Young, Old) |
Age Effect within Sex Effect within Genotype (C57BL/6J, Jimbee) |
|||||
| 16-18-week mice | 49-50-week mice | ||||||||
| C57BL/6J (n=10) |
Jimbee (n=11) |
C57BL/6J (n=12) |
Jimbee (n=21) |
||||||
| Tb.BV/TV | --- | 0.260 (0.021) | 0.230 (0.031) | 0.179 (0.015) | 0.171 (0.041) | NS | S | +0.017 [0.008], (NS) | +0.069 [0.008], (NS) |
| Sex within Geno.7 | −0.047 [0.008] | −0.015 [0.007] | |||||||
| Sex within Age 8 | −0.049 [0.008] | −0.013 [0.007]* | |||||||
| Conn.D | mm−3 | 239.6 (20.5) | 248.2 (39.0) | 159.6 (16.0) | 147.3 (46.1) | S | N/A9 | NS, (NS) | NS, (NS) |
| Sex within Geno. | −79.4 [11.0] | −27.8 [9.4] | |||||||
| SMI | --- | 0.69 (0.25) | 0.93 (0.31) | 1.19 (0.20) | 1.18 (0.40) | NS | S | NS, (NS) | −0.37 [0.08] |
| Sex within Age | NS | −0.25 [0.07] | |||||||
| Tb.N | mm−1 | 5.42 (0.21) | 5.29 (0.46) | 4.43 (0.51) | 4.23 (0.81) | NS | N/A | NS, (NS) | NS, (NS) |
| Sex within Geno. | −1.39 [0.19] | −0.74 [0.17] | |||||||
| Tb.Th | mm | 0.052 (0.003) | 0.048 (0.003) | 0.046 (0.004) | 0.046 (0.005) | N/A | S | NS, (NS) | +0.004 [0.001], (NS) |
| Sex within Age | NS | +0.004 [0.001] | |||||||
| Tb.Sp | mm | 0.18 (0.01) | 0.19 (0.02) | 0.22 (0.04) | 0.24 (0.06) | NS | N/A | NS, (NS) | NS, (NS) |
| Sex within Geno. | +0.10 [0.01] | +0.05 [0.01] | |||||||
| Tb.TMD | mgHA/cm3 | 980.8 (10.0) | 981.0 (8.7) | 996.0 (13.8) | 988.1 (15.4) | S | S | NS, N/A | −11.5 [3.9], N/A |
| Sex within Geno. | +6.7 [4.1]* | −6.2 [3.5]* | |||||||
| Sex within Age | −10.0 [4.2] | +10.5 [2.4] | |||||||
| VB strength | N | 36.5 (4.8) | 33.3 (3.7) | 24.9 (4.0) | 24.8 (6.3) | NS | S | NS, (NS) | +10.0 [1.3] |
| Sex within Age | −5.3 [1.5] | NS | |||||||
C57BL/6J LS means –Jimbee LS means.
16-18-weeks LS means – 49-50-weeks LS means.
C57BL/6 female LS means – Jimbee female LS means or C57BL/6 male LS means – Jimbee male LS means.
16-18-weeks C57BL/6J LS means – 16-18-weeks Jimbee LS means or 49-50-weeks C57BL/6J LS means – 49-50-weeks Jimbee LS means.
16-18-weeks female LS means −49-50-weeks female LS means or 16-18-weeks male LS means – 49-50-weeks male LS means.
16-18-weeks C57BL/6J LS means – 49-50-weeks C57BL/6J LS means or 16-18-weeks Jimbee LS means – 49-50-weeks Jimbee LS means.
female C57BL/6J LS means – male C57BL/6J LS means or female Jimbee LS means – male Jimbee LS means.
16-18-weeks female LS means – 16-18-weeks male LS means or 49-50-weeks female LS mean – 49-50-weeks male LS means.
Not applicable (N/A) because Genotype or Age did not interact with sex and/or Genotype did not interact with Age and so the difference in the LS means is the same for females and males.