| Antibodies |
|
|
| Peripherin |
Abcam |
ab4666; RRID: AB_449340 |
| MAP2 |
Abcam |
ab5392; RRID: AB_2138153 |
| NEFH |
Abcam |
ab72996; RRID: AB_2149618 |
| TUJ1 (mouse) |
Abcam |
ab78078; RRID: AB_2256751 |
| TUJ1 (chicken) |
Abcam |
ab107216; RRID: AB_10899689 |
| NeuN |
Abcam |
ab104225; RRID: AB_10711153 |
| BRN3A |
Millipore |
MAB1585; RRID: AB_94166 |
| ISL1 |
DSHB |
40.2D6; RRID: AB_528315 |
| TRKA |
Abcam |
ab76291; RRID: AB_1524514 |
| TRKB |
Alomone Labs |
ANT-019-AG; RRID: AB_10917156 |
| TRKC |
OriGene |
TA336908 |
| OCT4 |
Abcam |
ab19857; RRID: AB_445175 |
| SSEA4 |
STEMCELL Technologies |
60062 |
| NANOG |
ReproCELL |
RCAB0004P-F; RRID: AB_567470 |
| TRA-1–81 |
Millipore |
MAB4381; RRID: AB_177638 |
| SOX2 |
Thermo Fisher |
PA1–16968; RRID: AB_2195781 |
| TRA-1–60 |
Millipore |
MAB4360; RRID: AB_2119183 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| Y-27632 |
Tocris |
1254 |
| Doxycycline |
Clontech |
631311 |
| BDNF |
R&D Systems |
248BD025 |
| GDNF |
R&D Systems |
212GD010 |
| β-NGF |
R&D Systems |
256GF100 |
| NT-3 |
R&D Systems |
267N3025 |
| bFGF |
R&D Systems |
233FB025 |
| EGF |
R&D Systems |
236EG200 |
| SB431542 |
Tocris |
1614 |
| Retinoic acid |
Sigma-Aldrich |
R2625 |
| AITC |
Sigma-Aldrich |
377430 |
| Capsaicin |
Sigma-Aldrich |
M2028 |
| Menthol |
Sigma-Aldrich |
M2772 |
| α,β-meATP |
Sigma-Aldrich |
M6517 |
| RQ-00203078 |
Sigma-Aldrich |
SML1602 |
| Tetrodotoxin |
Tocris |
1078 |
| Critical Commercial Assays |
|
|
| Taqman Gene Expression Assay |
Thermo Fisher |
18080085 |
| Taqman RT-qPCR probes (see Oligonucleotides below) |
Thermo Fisher |
N/A |
| RNAscope® Fluorescent Multiplex Detection Reagents |
ACD |
320851 |
| RNAscope® Probe – Hs-TRPM8 |
ACD |
543121 |
| RNAscope® Probe – Hs-TRPM8-C3 |
ACD |
543121-C3 |
| RNAscope® Probe – Hs-PIEZO2 |
ACD |
449951 |
| RNAscope® Probe – Hs-PIEZO2-C2 |
ACD |
449951-C2 |
| RNAscope® Probe – Hs-NTRK1 |
ACD |
402631 |
| RNAscope® Probe – Hs-NTRK2-C2 |
ACD |
402621-C2 |
| RNAscope® Probe – Hs-NTRK3-C3 |
ACD |
406341-C3 |
| RNAscope® Probe – Hs-TUBB3-C2 |
ACD |
318901-C2 |
| Deposited Data |
|
|
| Bulk RNA sequencing data |
https://www.ncbi.nlm.nih.gov/geo/ |
Accession GSE139273
|
| Single-nuclei RNA sequencing data |
https://www.ncbi.nlm.nih.gov/geo/ |
Accession GSE139409
|
| Experimental Models: Cell Lines |
|
|
| WTC11 iPSC |
Miyaoka et al., 2014 |
Coriell GM25256 |
| WTC11 NGN2 iPSC |
Wang et al., 2017 |
N/A |
| WTC11 NGN2-BRN3A iPSC (C1) |
This study |
N/A |
| WTC11 NGN2-BRN3A iPSC, PIEZO2 knockout (KO) |
This study |
N/A |
| PIEZO2 patient 1 NGN2-BRN3A iPSC (P1) |
This study |
N/A |
| PIEZO2 patient 1 healthy sibling NGN2-BRN3A iPSC (C2) |
This study |
N/A |
| PIEZO2 patient 2 NGN2-BRN3A iPSC (P2) |
This study |
N/A |
| PIEZO2 patient 2 NGN2-BRN3A iPSC, CRISPR-Cas9-corrected (P2C) |
This study |
N/A |
| Oligonucleotides |
|
|
| CLYBL wild type genotyping primer, forward: 5′-TGACTAAACACTGT GCCCCA-3′ |
This study |
N/A |
| CLYBL wild type genotyping primer, reverse: 5′-AGGCAGGATGAAT TGGTGGA-3′ |
This study |
N/A |
| CLYBL 5′ insert genotyping primer, forward: 5′-CAGACAAGTCAGT AGGGCCA-3′ |
This study |
N/A |
| CLYBL 5′ insert genotyping primer, reverse: 5′-AGAAGACTTCCTCT GCCCTC-3′ |
This study |
N/A |
| CLYBL 3′ insert genotyping primer, forward: 5′-GGTAGGAAGTGGT ACGGAAA-3′ |
This study |
N/A |
| CLYBL 3′ insert genotyping primer, reverse: 5′-GAACGATTTACTGG GCAGTC-3′ |
This study |
N/A |
|
NGN2-BRN3A transgene RT-PCR primer, forward: 5′-CAAGATC GAAACCCTGAGAT-3′ |
This study |
N/A |
|
NGN2-BRN3A transgene RT-PCR primer, reverse: 5′-GATGGGTAC TTATGCTCAGG-3′ |
This study |
N/A |
|
PIEZO2 exon 32 sequencing primer, forward: 5′-AGACCCAATAT GCCAACACC-3′ |
Chesler et al., 2016 |
N/A |
|
PIEZO2 exon 32 sequencing primer, reverse: 5′-GGGAGCAGGCA TCATTACAA-3′ |
Chesler et al., 2016 |
N/A |
|
PIEZO2 exon 35 sequencing primer, forward: 5′-GGTAAAACATC GCTGGGCTA-3′ |
Chesler et al., 2016 |
N/A |
|
PIEZO2 exon 35 sequencing primer, reverse: 5′-AAGGGGTTATG CCACAACTG-3′ |
Chesler et al., 2016 |
N/A |
| RT-qPCR primer - RPLP0 |
IDT |
Hs.PT.39a.22214824 |
| RT-qPCR primer - ISL1 |
Thermo Fisher |
Hs00158126_m1 |
| RT-qPCR primer - POU4F1 |
Thermo Fisher |
Hs00366711_m1 |
| RT-qPCR primer - NTRK1 |
Thermo Fisher |
Hs01021011_m1 |
| RT-qPCR primer - NTRK2 |
Thermo Fisher |
Hs00178811_m1 |
| RT-qPCR primer - PIEZO1 |
Thermo Fisher |
Hs00207230_m1 |
| RT-qPCR primer - PIEZO2 |
Thermo Fisher |
Hs00926218_m1 |
| RT-qPCR primer - SCN9A |
Thermo Fisher |
Hs00161567_m1 |
| RT-qPCR primer - SCN10A |
Thermo Fisher |
Hs01045137_m1 |
| RT-qPCR primer - RET |
Thermo Fisher |
Hs01120030_m1 |
| RT-qPCR primer - MAF |
Thermo Fisher |
Hs04185012_s1 |
| RT-qPCR primer - PVALB |
Thermo Fisher |
Hs00161045_m1 |
| RT-qPCR primer - TRPV1 |
Thermo Fisher |
Hs00218912_m1 |
| RT-qPCR primer - TRPM8 |
Thermo Fisher |
Hs01066596_m1 |
| RT-qPCR primer - SOX10 |
Thermo Fisher |
Hs00366918_m1 |
| RT-qPCR primer - PAX3 |
Thermo Fisher |
Hs00240950_m1 |
| RT-qPCR primer - POU5F1 |
Thermo Fisher |
Hs00999632_g1 |
| RT-qPCR primer - NANOG |
Thermo Fisher |
Hs02387400_g1 |
| Recombinant DNA |
|
|
| pUCM-CLYBL-NGN2-BRN3A |
This study |
N/A |
| pZT-C13-L1 |
Cerbini et al., 2015 |
Addgene #62196 |
| pZT-C13-R1 |
Cerbini et al., 2015 |
Addgene #62197 |
