Figure 3.

Lycium barbarum polysaccharides (LBP) inhibits apoptosis of RGC-5 cells induced by cobalt chloride (CoCl₂).

Cells were treated with LBP (0.5 mg/mL) for 24 hours and then incubated with 200 μM of CoCl₂ for 24 hours. Apoptosis in RGC-5 cells treated with LBP was analyzed by flow cytometry. The cells were stained with enhanced green fluorescent protein-conjugated Annexin V and propidium iodide. The enhanced green fluorescent protein and PI fluorescence was measured using flow cytometer with Fluorescein-A and propidium iodide-A filter. (A) Representative dot plots of Annexin V/PI staining. The lower left quadrant contains the vital (double negative) population. (B) Quantitative result of early apoptotic cells. (C) Quantitative result of total apoptotic cells. Data were presented as the mean ± SD (n = 5) and analyzed by one-way analysis of variance followed by Student-Newman-Keuls test. **P < 0.01.