Figure 6.

Activation of mitophagy‐upstream kinase PINK1, mitophagy proteins, and Parkin is decreased in gas7‐knockout mouse cerebrum. A, Western blot analysis. Total brain lysates from wild‐type (WT), gas7 heterozygous (HT), and gas7‐knockout (KO) mice were electrophoresed by 10% SDS‐PAGE. After blotting, the membranes were probed with Gas7 antibody to show that Gas7 protein exists in wild‐type and gas7 heterozygotes, but not in gas7‐knockout mice. Blots show decreased PINK1, phospho‐Parkin (S65), Ubiquitin, and phospho‐Ubiquitin (S65) proteins in HT/KO mice. There were no significant differences for Parkin protein levels among WT, HT, or KO mice. GAPDH was used as a loading control. Quantification analysis of PINK1 (A1), phospho‐Parkin (A2), Ubiquitin (A4), phospho‐Ubiquitin (A5), and normalized phospho‐Ubiquitin (S65) over normalized Ubiquitin (A6) shows decreases in PINK1, phospho‐Parkin, Ubiquitin, and phospho‐Ubiquitin in KO mice. Actin and GAPDH were used as loading controls, for which there were no significant differences among WT, HT, or KO mice