Figure 7.

gas7‐knockout mice exhibit decreased energy consumption, respiration, and wheel speed, as well as increased body mass. A, Box plot analysis of average energy consumption by Gas7 wild‐type (WT, green) and ‐knockout (KO, blue) male mice, showing decreased average energy consumption of gas7 KO mice during both the dark (left side) and light (right side) cycles. B, Box plot analysis of maximum energy consumption for Gas7 WT (green) and KO (blue) male mice, revealing decreased maximum energy consumption of gas7 KO mice during both dark (left side) and light (right side) cycles. C, Box plot analysis of average oxygen inhalation by Gas7 WT (green) and KO (blue) male mice, showing decreased average oxygen inhalation of gas7 KO mice during both the dark (left side) and light (right side) cycles. D, Box plot analysis of differences in average CO₂ exhalation for Gas7 WT (green) and KO (blue) male mice, demonstrating a decrease in average CO₂ exhalation of gas7 KO mice during both the dark (left side) and light (right side) cycles. E, Box plot analysis of body mass differences for Gas7 WT (green) and KO (blue) male mice, showing increased body mass of gas7 KO mice during both the dark (left side) and light (right side) cycles. F, Box plot analysis of differences in wheel speed for Gas7 WT (green) and KO (blue) male mice, revealing a decrease in wheel speed of gas7 KO mice during both the dark (left side) and light (right side) cycles. G, Western blot analysis. Total brain lysates from wild‐type (WT), gas7 heterozygous (HT), and gas7‐knockout (KO) mice were electrophoresed by 10% SDS‐PAGE. After blotting, membranes were probed with phospho‐PDH (S293) and pyruvate dehydrogenase (PDH) antibodies. Blots show decreased levels of phospho‐PDH and PDH in HT and KO mice. GAPDH was used as a loading control. H, Quantification analysis of phospho‐PDH normalized to GAPDH levels, showing the decrease in PDH phosphorylation. I, Quantification analysis of PDH normalized to GAPDH levels, showing the decrease in levels of PDH. J, Quantification analysis of normalized phospho‐PDH over normalized PDH levels, showing phosphorylation levels of PDH increase significantly among WT, gas7 HT, and gas7 KO mice