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. 2020 Feb 28;7:55. doi: 10.3389/fmed.2020.00055

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Copyright © 2020 Franck, Ghozzi, Pajaud, Lawson-Hogban, Mas, Lacout and Perronne.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Several sequences of the glpQ gene portion used for the detection of B. miyamotoi and belonging to other species of the recurrent fever group of Borreliae are aligned and compared. Bold and underlined: primers for the target B. miyamotoi. The glpQ gene, in the current state of the art and genome annotations has not been described in Borrelia species other than those of the recurrent fever group. Highlighted: the sequence differences of some recurrent fever Borrelia compared to that of B. miyamotoi. The following sequences are aligned: B. miyamotoi (D43777.1), B. hermsii (DQ855539.1), B. lonestari (AY368275.1), B. duttoni (DQ346787.1), B. microti (EU914144.1), B. recurrentis (DQ346781) .1), B. turcica (AB529430.1), B. theileri (KF569938.1), B. persica (AY530742.1), B. venezuelensis (MG651651.1), B. hispanica (GU357572.1), B. parkeri (MH704900.1), B. crocidurae (JX292940.1). The regions of the primers are very different between species.