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. 2020 Feb 28;7:55. doi: 10.3389/fmed.2020.00055

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Copyright © 2020 Franck, Ghozzi, Pajaud, Lawson-Hogban, Mas, Lacout and Perronne.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Graph of the percentage of identical base pairs for the detected and undetected samples for B. miyamotoi PCR. The percentage of identical sequenced bases is >60% for the detected samples which display long runs of identical bases (B) and <20% for the samples not detected in PCR with no long runs of identical nucleotides (C). The sequence of interest of B. miyamotoi is a very short sequence (94bp). When sequencing small sequences, the first bases may not be recognized by the sequencer because of the brevity of the sequence, which explains why for some positive samples, sequencing only returns 60% of the bases of the sequence.