Table 2.
Characteristics of the B. miyamotoi PCR kit.
PCR Mix | LOD | LOQ | Repeatability | Reproducibility | ||||
---|---|---|---|---|---|---|---|---|
Tm | Mean efficacy | GU/PCR | GU/ml | GU/PCR | GU/ml | Mean CV | Mean CV | |
Borrelia miyamotoi | 79.5°C | 106.3% | 12.5 | 1,041 | 18.8 | 1,567 | 0.85 | 1.22 |
Tm, melting temperature; LOD, limit of detection; LOQ, limit of quantification; GU, genome unit; CV, coefficient of variation.