TABLE 1.

Activities versus different substrates and half-lives of different PFL biocatalysts prepared under different immobilization conditions.

Immobilization conditions	Activity versus pNPB (U/g)	Activity versus triacetin (U/g)	Half-life (min)
5 mM sodium acetate buffer, pH 5.0	13.25 ± 0.73	2.98 ± 0.14	90 ± 10.8
5 mM sodium acetate buffer, 100 mM NaCl, pH 5.0	13.42 ± 0.79	3.20 ± 0.17	120 ± 16.8
5 mM sodium acetate buffer, 250 mM NaCl, pH 5.0	13.47 ± 0.81	3.20 ± 0.19	140 ± 18.2
5 mM sodium acetate buffer, 30% glycerol, pH 5.0	13.83 ± 0.65	3.46 ± 0.17	110 ± 16.5
5 mM Tris buffer, pH 7.0	10.90 ± 0.62	2.68 ± 0.14	270 ± 37.8
5 mM Tris buffer, 100 mM NaCl, pH 7.0	11.67 ± 0.66	3.10 ± 0.18	180 ± 23.4
5 mM Tris buffer, 250 mM NaCl, pH 7.0	13.63 ± 0.78	3.30 ± 0.19	175 ± 22.8
5 mM Tris buffer, 30% glycerol, pH 7.0	12.79 ± 0.70	3.27 ± 0.18	240 ± 28.8
5 mM Tris buffer, 10 mM CaCl2, pH 7.0	13.86 ± 0.85	4.11 ± 0.23	280 ± 35.2
5 mM sodium phosphate buffer, pH 7.0	13.27 ± 0.77	3.42 ± 0.20	100 ± 13.6
100 mM sodium phosphate buffer, pH 7.0	13.17 ± 0.65	3.48 ± 0.18	220 ± 27.5
250 mM sodium phosphate buffer, pH 7.0	14.20 ± 0.85	3.94 ± 0.19	410 ± 60.5
5 mM sodium bicarbonate buffer, pH 9.0	13.97 ± 0.75	3.27 ± 0.16	140 ± 18.2
5 mM sodium bicarbonate buffer, 100 mM NaCl, pH 9.0	13.39 ± 0.79	3.10 ± 0.17	165 ± 22.4
5 mM sodium bicarbonate buffer, 250 mM NaCl, pH 9.0	16.29 ± 0.82	3.52 ± 0.18	155 ± 20.5
5 mM sodium bicarbonate buffer, 30% glycerol, pH 9.0	13.14 ± 0.72	2.58 ± 0.13	160 ± 21.8

The experiments were performed using biocatalysts having 1 mg of enzyme/g of support. The activities versus pNPB were measured as described in section “Materials and methods” at pH 7.0 and 25°C. The inactivations were performed at 70°C using 50 mM of Tris buffer at pH 7.0. Other specifications are described in section “Methods.”