Table 2.
Comparison of claudin expression data in brain endothelial cells or capillaries derived from the present work (hCMEC/D3), from Berndt et al. [3] (laser-microdissected human brain capillaries), the Barres1 (isolated brain endothelial cells) and the Betsholtz data base2 (single cell sequences brain capillary endothelial cells—capEC)
| Species | Current work qPCR3 | Rank | Berndt et al. [3] qPCR4 |
Rank | Barres data base (FPKM) | Rank | Betsholtz data base (counts) | Rank |
|---|---|---|---|---|---|---|---|---|
| Human | Human | Mouse | Mouse | |||||
| Cldn1 | 1000 | 2 | 30 | 4 | 0.2 | 7 | nd | – |
| Cldn2 | nd | – | 1.26 | 15 | 0.1 | 11 | 3 | 4 |
| Cldn3 | 5.2 | 20 | 6.08 | 9 | 0.3 | 5 | 0.05 | 9 |
| Cldn4 | 18 | 16 | 4.43 | 11 | 0.1 | 11 | nd | – |
| Cldn5 | 39 | 9 | 205 | 1 | < 3500 | 1 | 1500 | 1 |
| Cldn6 | 18 | 15 | 7.51 | 7 | 2 | 3 | 0.1 | 7 |
| Cldn7 | 219 | 5 | nd | – | 0.12 | 9 | nd | – |
| Cldn8 | 18 | 14 | nd | – | 0.1 | 11 | nd | – |
| Cldn9 | 25 | 12 | 13.4 | 5 | 0.15 | 8 | nd | – |
| Cldn10 | 142, 116 | 6a | nd | – | nd | – | nd | – |
| Cldn11 | 3189 | 1 | 162 | 2 | nd | – | nd | – |
| Cldn12 | 281, 18, 291 | 3, 4, 13b | 114 | 3 | 5 | 2 | 20 | 3 |
| Cldn13 | # | – | # | – | 0.1 | 11 | nd | – |
| Cldn14 | 69 | 7 | nd | – | nd | – | nd | – |
| Cldn15 | 17 | 17 | 6.13 | 8 | 0.4 | 4 | nd | – |
| Cldn16 | 8 | 19 | nd | – | 0.1 | 11 | 0.02 | 10 |
| Cldn17 | 30 | 10 | 5.85 | 10 | 0.1 | 11 | nd | – |
| Cldn18 | 27, 3.71 | 11, 22c | nd | – | 0.1 | 11 | 0.1 | 7 |
| Cldn19 | nd | – | nd | – | 0.1 | 11 | < 0.1 | 11 |
| Cldn20 | 52 | 8 | 3.35 | 13 | 0.1 | 11 | 0.4 | 5 |
| Cldn21 | nd | – | nd | – | ## | – | ## | – |
| Cldn22 | 9.97 | 18 | 3.95 | 12 | 0.3 | 5 | 0.2 | 6 |
| Cldn23 | 0.25 | 24 | 1.59 | 14 | 0.1 | 11 | nd | – |
| Cldn24 | 3.65 | 23 | nd | – | 0.12 | 9 | na | – |
| Cldn25 | 4.77 | 21 | nd | – | na | – | 30 | 2 |
nd = not detected; CLDN21 = CLDN22 at human; na = not available; #: not encoded in human; ##: not encoded in mouse; adata listed are for Cldn10 tva and tvb; bdata listed are for Cldn12 tv1, tv2, tv3; cdata listed are for Cldn18 tv1b and tv2a.; 1Zhang et al. [54], accessed on 21st of January 2020: https://web.stanford.edu/group/barres_lab/brain_rnaseq.html; 2Vanlandewijck et al. [46], He et al. [16], accessed on 21st of January 2020: http://betsholtzlab.org/VascularSingleCells/database.html; 3mean values related to Cldn1, which was set to 1000; 4mean values * 10−4 from the supplementary file of Berndt et al. [3]