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. 2020 Mar 6;46:29. doi: 10.1186/s13052-020-0792-x

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© The Author(s). 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — ROS production evaluation and platelet characteristics in patients with bronchiolitis (15 boys and 12 girls). a ROS levels measured in fresh whole blood by electron paramagnetic resonance. ##/°°p < 0.01 (Patients vs HCs). The measures were carried out in triplicate. Histograms showing the percentage of platelets positives to: b Annexin V; c P-selectin/CD62; d PAC-1. Measures carried out by flow cytometry. The values represent the percentage ± SD of positive cells. Statistical analysis was performed by one-way analysis of variance (ANOVA). # p < 0.05 (Boys patients vs Boys HC); ## p < 0.01(Boys patients vs Boys HC); °°p < 0.01 (Girls patients vs Girls HC); °°°p < 0.001 (Girls patients vs Girls HC); §p < 0.05 (Boys patients vs Girls patients); §§p < 0.01 (Boys patients vs Girls patients). On the right typical flow cytometric measures of a representative HC boy and a representative patient boy, and a representative HC girl and a representative patient girl. (Pt = patient; HC = control group)