Table 2.
List of primers used in this study
| PCR | Primer | Sequence (5′-3′) | Product size (bp) | PCR cycles | Annealing temperature (°C) |
|---|---|---|---|---|---|
| qRT-PCR | GAPDHF | GGAGTCAACGGATTTGGT | 206 | 23 | 55 |
| GAPDHR | GTGATGGGATTTCCATTGAT | ||||
| SEPT9_v2F | TGGGATCATTTCGGACTTCG | 167 | 60 | ||
| SEPT9_v2R | AGGGTTCTGAGTTCTTCACG | ||||
| MMP1F | GAGATCATCGGGACAACTCTCCTT | 119 | |||
| MMP1R | GTTGGTCCACCTTTCATCTTCATCA | ||||
| MMP10F | AGACACTGACACTCTGGAGG | 202 | |||
| MMP10R | TGGAGTCACCTCTTCCCAGA | ||||
| FZD10F | ATCGGCACTTCCTTCATCCTGTC | 199 | |||
| FZD10R | TCTTCCAGTAGTCCATGTTGAG | ||||
| miR-92b-3pF | TATTGCACTCGTCCCG | ||||
| miR-92b-3PR | GTGCAGGGTCCGAGGT | ||||
| miR-92b-3PRT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGAGGC | ||||
| U6F | CTCGCTTCGGCAGCACA | ||||
| U6R | AACGCTTCACGAATTTGCGT | ||||
| U6RT | AACGCTTCACGAATTTGCGT | ||||
| GAPDHF | CCAGCAAGAGCACAAGAGGAA | 114 | |||
| GAPDHR | CAAGGGGTCTACATGGCAACT | ||||
| MSP | SEPT9_v2m1 | GGTTTAGGGGTTTTTTCGGC | 137 | 60 | |
| SEPT9_v2m2 | AACTAATAAACAACGAATCGCG | ||||
| SEPT9_v2u1 | GGGTTTAGGGGTTTTTTTGGT | 141 | 58 | ||
| SEPT9_v2u2 | AATAACTAATAAACAACAAATCACA |