Table 2.

Primer sets for multilocus sequence typing (MLST) analysis of Golovinomyces in this study

DNA regions	Primer	Primer sequences (5′ → 3′)	Annealing temperature (°C)	Amplicon size (bp)	Reference
ITS	ITS5 ITS4	GGAAGTAAAAGTCGTAACAAGG TCCTCCGCTTATTGATATGC	52	600	[44]
28S rDNA	LSU1 LSU2	ACCCGCTGAACTTAAGCATA CCTTGGTCCGTGTTTCAAGA	52	500	[45]
IGS	IGS-12a NS1R	AGTCTGTGGATTAGTGGCCG GAGACAAGCATATGACTAC	52	400	[46]
TUB2	TubF1 TubR1	AGGTTCACCTCCAGACTGG CCAGCACGAACAGCATCCAT	52	450	This study
CHS1	gCS1a1 gCS1b	GGTGCATTCTCGGCATATCG CGTCACCCTTGGTGCCCCAAG	52	1000	This study