Skip to main content
. 2020 Feb 28;11:88. doi: 10.3389/fphar.2020.00088

Figure 3.

Figure 3

Compound C inhibited the dsDNA-dependent pathway which was not dependent on the activity of AMP-activated protein kinases (AMPKs). (A) Western blot analyses of phorsphorylated AMPK in L929 wild-type and L929-AMPK-/- cells. The cells were transfected with HT-DNA and poly (I:C). (B, C) RT-qPCR analyses of IFNβ expression in L929 and L929-AMPK-/- cells. The cells were transfected with HT-DNA (B) or poly (I:C) (C) after treated with indicated dose of Compound C. (D–F) RT-qPCR analyses of IFNβ and CXCL10 expression in L929 wild-type and L929-AMPK-/- cells. The cells were transfected HT-DNA (D), cGAMP (E); Analyses of IFNβ expression in L929 wild-type and L929-AMPK-/- cells. The cells were transfected with poly (I:C) (F). The experiments were performed at least three times. Data in bar graphs are presented as mean ± SD (n = 5) with asterisks indicating significant changes between the indicated bars (NS, Nonsignificance, * P < 0.05, **P < 0.01,***P < 0.001).