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. 2020 Feb 28;11:88. doi: 10.3389/fphar.2020.00088

Figure 5.

Figure 5

Compound C lowered the cGAMP level. (A) RT-qPCR analyses of IFNβ induction in THP1-ISG-liciferase cells. The cells were treated with the increasing amounts of cGAMP. (B) RT-qPCR analyses of IFNβ induction in THP1-ISG-liciferase cells. The cells were treated with the S100 fractions which were extracted from the BJ cells transfected with HT-DNA after treated with indicated doses of Compound C for 1h. (C, D) RT-qPCR analyses of IFNβ (C), CXCL10 (D) expression in THP1-ISG-liciferase cells treated with the S100 fractions which were extracted from the BJ cells transfected with HT-DNA after treated with indicated doses of Compound C for 1h. (E, F) LC-MS analyses of the content of cGAMP in the S100 fractions which were extracted from the BJ cells transfected with HT-DNA after treated with 10 μM Compound C. The experiments were performed at least three times. Data in bar graphs are presented as mean ± SD (n = 3−5) with asterisks indicating significant changes (NS, Nonsignnificant, * P < 0.05, **P < 0.01,***P < 0.001).