Fig. 3.

Effects of pH and HC-3 treatment on [³H]choline uptake in LNCaP cells. (A) Influence of extracellular pH on [³H]choline uptake in LNCaP cells. [³H]choline uptake (10 μM) was measured for 20 min under different pH conditions. Data are presented as a percentage of uptake relative to that at pH 7.5. ^*p<0.05, ^**p<0.01, and ^***p<0.001 compared to pH 7.5. (B) Effect of HC-3 on [³H]choline uptake in LNCaP cells. Cells were pre-incubated for 20 min with increasing concentrations of HC-3 followed by the measurement of [³H]choline uptake for 20 min. Results are shown as a percentage of the uptake for the vehicle control. All data are presented as the mean ± SD (n=4).