Fig. 5.

Effect of bicalutamide on cell viability, [³H]choline uptake, and caspase-3/7 activity in LNCaP cells. (A) Cell viability was measured 24 h after treatment with increasing concentrations of flutamide. The results are presented as the percentage of the vehicle control. (B) LNCaP cells were incubated for 20 min with increasing concentrations of bicalutamide followed by the measurement of [³H]choline uptake. [³H]Choline uptake was calculated as uptake per protein. The results are presented as the percentage of uptake in the vehicle control sample. (C) Cells were incubated for 24 h with increasing concentrations of bicalutamide followed by the measurement of caspase-3/7 activity and cell number. Caspase activity was calculated as the activity per cell number. The results are shown as a percentage of the vehicle control. (D) Correlation analysis between inhibition of [³H]choline uptake and cell viability for LNCaP cells treated with increasing concentrations of bicalutamide. The correlation was highly statistically significant (p=0.0075) with a correlation coefficient of R square=0.9329. Data for all assays are presented as the means ± SD (n=4). ^**p<0.01 compared with vehicle.