Fig. 4.

Amentoflavone directly disrupts the fibrillar structure of preformed Aβ1–42 fibrils. (A) Representative images taken by atomic force microscopy (AFM). Preformed Aβ1–42 fibrils was incubated in the absence or in the presence of an equal concentration (25 μM) of amentoflavone (AF), and subjected to AFM imaging. Scale bar indicates 200 nm. (B, C) Representative images of Western immunoblotting. The reaction samples from the experiment A were separated by SDS-PAGE in denatured condition (B) or after cross-linking using glutaraldehyde (C), followed by immunoblotting with a monoclonal anti-Aβ antibody, 6E10. Representative images from three independent experiments are presented. The intensity of the signals corresponding to Aβ fibrils (M.W.: ≥75 kDa), oligomers (M.W.: 8–30 kDa) and monomers (M.W.: 4 kDa) were quantified and presented as relative ratio to the fibrils. Data indicate mean ± standard error of mean (n= 4). n.s.: not significant (p>0.05) analyzed by t-test.