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. 2020 Feb 28;11:183. doi: 10.3389/fphar.2020.00183

Figure 5.

Figure 5

Ligands of mGlu2/3 receptors regulate expression of polo-like kinase 2 (PLK2) and phosphorylated α-synuclein at Ser129 in substantia nigra (SN) of sub-acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson’s disease (PD) mice model. Western blots represent the expression of PLK2 (A) and pS129 α-synuclein (B) in SN of each group. The band intensity of particular target proteins was normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) or β-actin level (C, D). * p < 0.05, ** p < 0.01; # p < 0.05 vs. LY354740 + saline group. N = 3 in (C); N = 4 in (D). Data were expressed as % of the normal + saline group and shown as the mean ± SEM. Immunoblots (G) and corresponding histograms (H) show expression of PLK2 and pS129 α-synuclein in SN. && p < 0.01 compared with other two groups; * p < 0.05. N = 3. Data were expressed as % of the MPTP + saline group and shown as the mean ± SEM. (E, F) Immunofluorescence and analysis of PLK2 positive cell counts in SN. Scale bar = 50 µm. * p < 0.05, ** p < 0.01 compared with Normal+Saline group; # p < 0.05 compared with MPTP+LY354740 group. N = 3. Data were shown as the mean ± SEM.