Fig. 3.

ABT-263 reduces MLN8237-induced polyploidy and increases death in mitosis. (A) Cells were treated with 0.5 µM MLN8237 +/− ABT-263 (1 µM), and DNA content was quantified by PI uptake. Representative cell cycle profiles are displayed and quantitative data represent the mean ± SEM (N ≥ 3). (B) Alterations in cellular ploidy were quantified following cell cycle experiments, KNS42 (72 h) and DAOY (48 h) (N ≥ 3): *P ≤ 0.05 MLN8237/ABT-263 versus MLN8237 alone. (C) Cells were imaged for 72 h by time-lapse microscopy. A minimum of 50 cells per condition were analyzed.