Table 1.
Pairwise comparisons of the treatment effect (1-MT vs. control) using Tukey-Kramer test.
| 1-MT | Control | SE | P-value | |
|---|---|---|---|---|
| LSM | LSM | |||
| TRP (μM) | ||||
| Balb/C | 250.94 | 227.44 | 3.24 | 0.003 |
| IDO1−/− | 221.61 | 202.00 | 4.43 | 0.030 |
| Human | 220.78 | 197.00 | 5.08 | 0.002 |
| KYN (μM) | ||||
| Balb/C | 1.72 | 2.26 | 0.11 | 0.001 |
| IDO1−/− | 1.45 | 1.90 | 0.07 | 0.001 |
| Human | 1.62 | 2.34 | 0.18 | 0.001 |
| KYNA (μM) | ||||
| Balb/C | 4.95 | 4.06 | 1.05 | 0.047 |
| IDO1−/− | 2.56 | 1.20 | 0.16 | <0.001 |
| Human | 4.09 | 1.37 | 0.29 | <0.001 |
| QUIN (μM) | ||||
| Balb/C | 0.42 | 0.52 | 0.02 | 0.002 |
| IDO1−/− | 0.38 | 0.45 | 0.01 | <0.001 |
| Human | 0.33 | 0.32 | 0.01 | 0.64 |
| 5-HT (μM) | ||||
| Balb/C | 1.20 | 1.37 | 0.03 | <0.001 |
| IDO1−/− | 1.12 | 1.28 | 0.02 | <0.001 |
| Human | 1.01 | 1.08 | 0.01 | 0.002 |
| KYN/TRP ratio | ||||
| Balb/C | 0.69 | 1.00 | 0.06 | 0.001 |
| IDO1−/− | 0.66 | 0.94 | 0.03 | <0.001 |
| Human | 0.73 | 1.22 | 0.12 | 0.002 |
Concentrations of the metabolites TRP, KYN, KYNA, QUIN, 5-HT were measured in cell culture supernatants of isolated splencocytes (Balb/C, IDO1−/−) and human whole blood cell culture. As marker for IDO activity KYN/TRP ratio was calculated. Results are presented as LS-means (LSM) ± SE averaged over three incubation times (6, 24, 36 h) per subject. 1-MT: n = 18 (six per species); Control: n = 18 (six per species).
P-values < 0.05 are marked in bold.