Fig 1.

A) Percent methylation at the Kcnq1ot1 ICR in all individual embryonic placentas derived from young paternal males at 4–6 months (black dots; n = 48) compared to the same paternal males when aged (n = 48); subdivided into 11–12 months (grey dots; n = 30) and 14–15 months (red dots; n = 18). Bolded middle line denotes the mean; error bars represent standard deviation. B) Average percent methylation at the Kcnq1ot1 ICR for embryonic placentas from each of the eight young paternal males (n = 6/male) and the same paternal males when aged (n = 6/male), subdivided into 11–12 months (grey lines; n = 5 males) and 14–15 months (red lines; n = 3 males). C) Average percent methylation for 20 CpG sites within the amplified Kcnq1ot1 ICR from embryonic placentas derived from young paternal males (black dots; n = 48) compared to the same paternal males when aged (dark grey dots; n = 48), subdivided into 11–12 months (light grey dots; n = 30) and 14–15 months (red dots; n = 18). Error bars represent standard error of the mean. Statistical significance of p<0.05 is denoted by a star.