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. 2020 Mar 2;15:1397–1408. doi: 10.2147/IJN.S227289

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© 2020 Hou et al.

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(A and B) Cell uptake of FAM-siRNA to SGC-7901 (A) or A549 (B) cells mediated by NP or NPF measured by FCM. (C and D) Intracellular distribution of FAM-siRNA to SGC-7901 (C) or A549 (D) cells mediated by NP or NPF captured by CLSM. FA represented the cells pretreated with free FA to block the FA receptor on cell membrane.

Notes: Blue, DAPI; Green, FAM-labeled siPLK1. The scale bar is 50 µm.

Abbreviations: NP, N-acetyl- L-leucine-polyethylenimine; NPF, Folate- N-acetyl- L-leucine-polyethylenimine; FA, folate; FAM, 5-Carboxyfluorescein.