(
A) Genes differentially expressed between clonotypes. Gene expression in each cell was averaged over the clonotypes before differential gene expression analysis. Unsupervised clustering shows two clusters with very similar gene expression to clusters 1 and 2 observed on scRNAseq of individual cells (
Figure 5B). (
B) Frequency of clonotypes corresponding to cluster 1 and 2, after primary immunization (left), and 18 months later before the booster vaccination (right). Clonotypes associated to cluster one are significantly more abundant on both these timepoints (Mann Whitney U-test A: p-value=0.0003; B: p-value=0.02447 ). .