Key resources table.
| Reagent type | Designation | Source | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-CD3-FITC (Mouse monoclonal) | eBioscience | CAT# 11-0038-42 | FACS (5 ul per test) |
| Antibody | anti-CD45RA-eFluor450 (Mouse monoclonal) | eBioscience | CAT# 48-0458-42 | FACS (5 ul per test) |
| Antibody | anti-CCR7-AlexaFluor647 (Rat monoclonal) | BD Pharmingen | CAT# 560921 | FACS (5 ul per test) |
| Antibody | anti-CD95-PE (Mouse monoclonal) | eBioscience | CAT# 12-0959-42 | FACS (5 ul per test) |
| Antibody | anti-CD3-eFluor450 (Mouse monoclonal) | eBioscience | CAT# 48-0038-42 | FACS (5 ul per test) |
| Other | HLA-A*0201 (LLWNGPMAV) dextramer | Immudex | CAT# WB3584 | FACS (10 ul per test) |
| Commercial kit | Chromium Single Cell A Chip Kit | 10x Genomics | CAT# 1000009 | |
| Commercial kit | Chromium Next GEM 5’ Library and Gel Bead Kit | 10x Genomics | CAT# 1000014 | |
| Commercial kit | Chromium V(D)J Enrichment Kit, Human T Cell | 10x Genomics | CAT# 1000005 | |
| Commercial kit | Chromium Single Cell 5’ Library Construction Kit | 10x Genomics | CAT# 1000020 | |
| Commercial kit | Chromium i7 Multiplex Kit | 10x Genomics | CAT# 120262 | |
| Commercial kit | Dynabeads CD4 Positive Isolation Kit | Invitrogen | CAT# 11331D | |
| Commercial kit | Dynabeads CD8 Positive Isolation Kit | Invitrogen | CAT# 11333D |