Table 1. Data values and statistical analyses.

Columns represent (from left to right): figure/panel number; experimental conditions; number of samples (synapses, dishes and cultures); mean values and standard errors of the means (SEM); statistical test used for comparison; and the P-value resulting from the statistical comparison.

Figure number	Conditions	NSyn	NDishes	NCultures	Mean ± SEM	Statistical test	Pval
Figure 1D	All	3781	90	11	0.1014 ± 0.0009	Two-sample KS-test	<0.001
	MVR	245	90	11	0.164 ± 0.005
Figure 1E	All	3781	90	11	6.57 ± 0.05	Two-sample KS-test	<0.001
	MVR	245	90	11	9.8 ± 0.03
Figure 1G	Proximal	245	90	11	0.45 ± 0.05	Chi-square test	0.0386
	Distal	245	90	11	0.29 ± 0.05
Figure 1H	Distal MVR/UVR	66	90	11	1.24 ± 0.09	Paired t-test	0.006
	Proximal MVR/UVR	95	90	11	1.28 ± 0.08	Paired t-test	<0.001
Figure 2C	UVR	151	90	11	0.32 ± 0.02	Two-sample KS-test	<0.001
	MVR	144	90	11	0.47 ± 0.02
Figure 3B	Proximal MVR/Proximal MVR	245	90	11	1 ± 0.03	Chi-square test	0.017
	Distal MVR/Proximal MVR	245	90	11	0.65 ± 0.02
Figure 3C	Larger MVR	245	90	11	214 ± 7	Two-sample t-test	0.0058
	Smaller MVR	245	90	11	242 ± 7
Figure 3D	MVR	245	90	11	y = 3.108+0.02106 x	Linear fit	<0.001
Figure 3E	MVR <400 nm	129	90	11	9.8 ± 0.7	Two-sample t-test	<0.001
	MVR >400 nm	115	90	11	14.3 ± 0.9
Figure 4A	EGTA MVR Linear fit	225	57	11	y = 8.801+0.00556 ×	Linear fit	0.264
	MVR <400 nm	156	57	11	10.1 ± 0.8	Two-sample t-test	0.131
	MVR >400 nm	69	57	11	12.3 ± 1.2
Figure 4B	Ctrl MVR	245	90	11	0.021 ± 0.005	One-way analysis of covariance (ANOCOVA)	0.022
	EGTA MVR	225	57	11	0.006 ± 0.005
Figure 4C	Ctrl MVR	245	90	11	52 ± 3	Chi-square test	<0.001
	EGTA MVR	225	57	11	69 ± 3
Figure 4D	Larger MVR	225	57	11	178 ± 7	Two-sample t-test	<0.001
	Smaller MVR	225	57	11	216 ± 7
Figure 4E	Ctrl Pr = 0.042	2417	90	11	104 ± 8	Two-sample t-test	<0.001
	EGTA Pr = 0.042	2338	57	11	62 ± 5
	Ctrl Pr = 0.033	2417	90	11	93 ± 3	Two-sample t-test	<0.001
	EGTA Pr = 0.033	2338	57	11	72 ± 2
	Ctrl Pr = 0.025	2417	90	11	107 ± 2	Two-sample t-test	<0.001
	EGTA Pr = 0.025	2338	57	11	83 ± 1
	Ctrl Pr = 0.017	2417	90	11	124 ± 1	Two-sample t-test	<0.001
	EGTA Pr = 0.017	2338	57	11	100 ± 1
	Ctrl Pr = 0.008	2417	90	11	154.6 ± 0.7	Two-sample t-test	<0.001
	EGTA Pr = 0.008	2338	57	11	129.8 ± 0.8
Figure 4F	Ctrl MVR	2417	90	11	${\displaystyle y=a+b\ast x^c}$	Fit	0.0030
	EGTA MVR	2338	57	11	a = 17.951
					b = 1.0049e+09
					c = 5.5575
Figure 4G	Distal MVR/UVR	52	57	11	1.29 ± 0.09	Paired t-test	0.0020
	Proximal MVR/UVR	77	57	11	1.5 ± 0.1	Paired t-test	<0.001
Figure 3—figure supplement 1A	UVR	136	90	11	11.5 ± 0.8	Two-sample t-test	0.1262
	MVR,<400 nm	129	90	11	9.8 ± 0.7
	UVR	136	90	11	11.5 ± 0.8	Two-sample t-test	0.0232
	MVR,>400 nm	115	90	11	14.3 ± 0.9
Figure 3—figure supplement 1B	UVR	665	90	11	y = 35.088-0.0064674 x	Linear fit	0.207
	UVR 0–100 nm	285	90	11	34.9±0.6	Two-sample t-test	0.1376
	UVR 200–300 nm	109	90	11	33.4 ± 0.9
Figure 3—figure supplement 1D	Synaptic vesicle diameters	NSyn = 93 NVesic = 806	–	3	y = 48.109+0.0047331x	Linear fit	0.161
Figure 4—figure supplement 1A	Larger Ctrl MVR	245	90	11	214 ± 7	Two-sample t-test	0.0058
	Smaller Ctrl MVR	245	90	11	242 ± 7
	Larger EGTA MVR	225	57	11	178 ± 7	Two-sample t-test	<0.001
	Smaller EGTA MVR	225	57	11	216 ± 7
	Larger Ctrl MVR	245	90	11	214 ± 7	Two-sample t-test	<0.001
	Larger EGTA MVR	225	57	11	178 ± 7
	Smaller Ctrl MVR	245	90	11	242 ± 7	Two-sample t-test	0.0116
	Smaller EGTA MVR	225	57	11	216 ± 7
Figure 4—figure supplement 1B	Ctrl Pr = 0.042	2417	90	11	104 ± 8	Two-sample t-test	<0.001
	EGTA Pr = 0.042	2338	57	11	62 ± 5
	Ctrl Pr = 0.033	2417	90	11	93 ± 3	Two-sample t-test	<0.001
	EGTA Pr = 0.033	2338	57	11	72 ± 2
	Ctrl Pr = 0.025	2417	90	11	107 ± 2	Two-sample t-test	<0.001
	EGTA Pr = 0.025	2338	57	11	83 ± 1
	Ctrl Pr = 0.017	2417	90	11	124 ± 1	Two-sample t-test	<0.001
	EGTA Pr = 0.017	2338	57	11	100 ± 1
	Ctrl Pr = 0.008	2417	90	11	154.6 ± 0.7	Two-sample t-test	<0.001
	EGTA Pr = 0.008	2338	57	11	129.8 ± 0.8