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Gating strategy in BAT. Basophilic cells were selected from whole blood based on CCR3high/SSClow. Activation of basophils was determined by expression of an activation marker CD63. Expression of CD63 for unstimulated control, 2 stimulation controls (anti-FCεRI mAb and fMLP), and stimulation with 100 ng/mL cod parvalbumin is demonstrated for P1 as an example. fMLP, Formyl-methionyl-leucyl-phenylalanine.
