FIG. 1.

Workflow of single-cell analysis. The biological sample is first sorted and enriched for the population of interest at the single-cell level. Each cell is then isolated in individual reaction chamber, followed by cell lysis to release biological materials. The materials, such as gDNA, mRNA, or expressed proteins, are processed depending on the target analysis, to downstream readouts, such as next-generation sequencing, or fluorescent signal as reporter.